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	Provedor de dados Naturalis (2) Autor Crespo, A. (1) Divakar, P.K. (1) Fankhauser, J.D. (1) Herman-Sackett, E. (1) Johnson, E.M. (1) Kalb, K. (1) Kandel, P. (1) Lumbsch, H.T. (1) Nelsen, M.P. (1) Nelson, N.A. (1) Rivas-Plata, E. (1) Schmitt, I. (1) Shimp, A.D. (1) Tretter, E.D. (1) Wang, Y. (1) White, M.M. (1) Widhelm, T. (1) Mais... Palavra-chave DNA replication licensing factor (2) MS277 (2) MS456 (2) Ascomycota (1) Evolution (1) Harpellales (1) Kickxellomycotina (1) Lichenised fungi (1) MCM7 (1) Mcm7 (1) Phylogeny (1) Pre-rRNA processing protein (1) Protein-coding (1) Ribosomal biogenesis protein (1) TSR1 (1) Trichomycetes (1) Tsr1 (1) Zygomycota (1) Mais... Tipo do documento Article / Letter to the editor (2) Ano 2013 (1) 2009 (1) País Netherlands (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Examining new phylogenetic markers to uncover the evolutionary history of early-diverging fungi: comparing MCM7, TSR1 and rRNA genes for single- and multi-gene analyses of the Kickxellomycotina Naturalis Tretter, E.D.; Johnson, E.M.; Wang, Y.; Kandel, P.; White, M.M.. The recently recognised protein-coding genes MCM7 and TSR1 have shown significant promise for phylogenetic resolution within the Ascomycota and Basidiomycota, but have remained unexamined within other fungal groups (except for Mucorales). We designed and tested primers to amplify these genes across early-diverging fungal clades, with emphasis on the Kickxellomycotina, zygomycetous fungi with characteristic flared septal walls forming pores with lenticular plugs. Phylogenetic tree resolution and congruence with MCM7 and TSR1 were compared against those inferred with nuclear small (SSU) and large subunit (LSU) rRNA genes. We also combined MCM7 and TSR1 data with the rDNA data to create 3- and 4-gene trees of the Kickxellomycotina that help to resolve... Tipo: Article / Letter to the editor Palavras-chave: DNA replication licensing factor; Harpellales; Kickxellomycotina; MCM7; MS277; MS456; Ribosomal biogenesis protein; Trichomycetes; TSR1; Zygomycota. Ano: 2013 URL: http://www.repository.naturalis.nl/record/532223 New primers for promising single-copy genes in fungal phylogenetics and systematics Naturalis Schmitt, I.; Crespo, A.; Divakar, P.K.; Fankhauser, J.D.; Herman-Sackett, E.; Kalb, K.; Nelsen, M.P.; Nelson, N.A.; Rivas-Plata, E.; Shimp, A.D.; Widhelm, T.; Lumbsch, H.T.. Developing powerful phylogenetic markers is a key concern in fungal phylogenetics. Here we report degenerate primers that amplify the single-copy genes Mcm7 (MS456) and Tsr1 (MS277) across a wide range of Pezizomycotina (Ascomycota). Phylogenetic analyses of 59 taxa belonging to the Eurotiomycetes, Lecanoromycetes, Leotiomycetes, Lichinomycetes and Sordariomycetes, indicate the utility of these loci for fungal phylogenetics at taxonomic levels ranging from genus to class. We also tested the new primers in silico using sequences of Saccharomycotina, Taphrinomycotina and Basidiomycota to predict their potential of amplifying widely across the Fungi. The analyses suggest that the new primers will need no, or only minor sequence modifications to amplify... Tipo: Article / Letter to the editor Palavras-chave: Ascomycota; DNA replication licensing factor; Evolution; Lichenised fungi; Mcm7; MS277; MS456; Phylogeny; Pre-rRNA processing protein; Protein-coding; Tsr1. Ano: 2009 URL: http://www.repository.naturalis.nl/record/531799 Registros recuperados: 2 Primeira ... 1 ... Última

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